Expertise

  • Fungal molecular and cell biology


Languages

  • Hungarian mother tongue, English excellent, German and French basic


Education and Professional Experience

  • 1985    Masters in Biology and Chemistry, ELTE University Budapest
  • 1990 - 1991 graduate student, University of Bern, Switzerland
  • 1991 - 1995 PhD in Biochemistry, University of Fribourg, Switzerland
  • 1996 - 2002 Postdoctoral fellow, University of Michigan, Ann Arbor, USA
  • 2002 - 2003 Postdoctoral fellow, University of Leuven and VIB, Belgium
  • 2004 - 2006 Senior research fellow, University of Vienna, BioCenter Vienna, Austria
  • 2008    University of West Hungary


Courses

  • 2012 Spring Semester: Modern molecular biology applications in environmental microbiology


Selected Publications

  • Sipos, G., Puoti, A. & Conzelmann, A., (1994). GPI membrane anchors in Saccharomyces cerevisiae: absence of ceramides from complete precursor glycolipids.  EMBO Journal.  13,  2789-2796.
  • Sipos, G., Puoti, A. & Conzelmann, A., (1995). Biosynthesis of the side chain of yeast GPI anchors is operated by novel mannosyltransferases located in the ER and the Golgi apparatus. J. Biol. Chem.  270, 19709-19715.
  • Sipos, G., Reggiori, F., Vionnet, C., & Conzelmann, A., (1997). Alternative lipid remodeling pathways for glycosylphosphatidylinositol membrane anchors in S. cerevisiae.  EMBO Journal.  12, 3494-3505
  • Brickner, J., Giardini, P., Sipos, G., Brace, EJ., & Fuller, RS., (1999) Genetic analysis of the roles of Soi1p, Soi2p and Soi3p in the localization of TGN membrane proteins, Mol. Biol. Cell: 9: (Suppl. S) 1896
  • Benachur, A., Sipos, G., Flury, I., Reggiori, F., Canivenc-Gansel, E., Vionnet, C., Conzelmann, A., & Benghezal, M. (1999) Deletion of GPI7, a yeast gene required for addition of a side chain to the glycosylphosphatidylinositol (GPI) core structure, affects GPI protein transport, remodeling and cell wall integrity. J. Biol. Chem.  274, 15251-15261
  • Brickner, J.H., Blanchette, J.M., Sipos, G. & Fuller, R.S.(2001) The Tlg SNARE complex is required for TGN homotypic fusion. J. Cell Biology 155/6, 969-978
  • Sipos, G. & Fuller, R. (2002) Separation of Golgi and endosomal compartments. (Review) Methods in Enzymology, Volume 351, 351-365
  • Sipos, G., Brickner, J.H, Brace, E.J., Chen, L., Rambourg, A., Kepes, F. & Fuller, R.S. (2004) Soi3/Rav1p functions at the early endosome to regulate endocytic trafficking to the vacuole and localization of TGN transmembrane proteins Mol. Biol Cell, Volume 15, 3196-3209
  • Sipos, G. & Kuchler, K. (2006) Fungal ATP-binding casette (ABC) transporters in drug resistance & cellular detoxification (Review) Current Drug Targets, Volume 7/4, 471-481

 


Recent activities


Research Interests

1. Identification of fungal virulence factors

Recently, we have sequenced the transcriptomes of virulent and non-virulent strains of the pathogenic Armillaria ostoyae grown under selective conditions (Sipos et al. Ms in preparation). Our main interests were to identify differentially expressed gene sets which might be involved in pathogenicity and to find further clues to how virulence could be regulated at the level of gene expression. Quantitative expression profiling and analysis of the potential role of splicing in virulence, through exon-based microarrasy, are under way.

 

2. Invasive and indigenous Phytophthora species in the Carpathian Basin

The soilborne Phytophthora (Stramenopila, Oomycota) species represent a group of highly invasive pathogens that are destroyers of native ecosystems, woodlands and agricultural settings worldwide. There are species, which appear to be indigenous as they live together with their hosts without a noticeable harm, while others, eluded from their native environment, can pose
devastating threats to hundreds of plant species in a new environment. The majority of the Phytophthora species have been identified from forest ecosystems.  We are interested to conduct a general survey of the forested areas in the Carpathian Basin.

 

3. The interactive secretomes of lichens

The aim is to identify the interactive secretomes of the symbiotic partners of Lobaria pulmonaria. My special interest in fungal molecular biology is to study how fungal secretory proteins (secretomes) are regulated to drive adaptive responses and speciation. Lobaria may provide us with a unique and very interesting system at the level of regulated secretomes. Direct interactions of the biotic partners are mediated by proteins, which are secreted as they are localized at the interacting surface. Such proteins could be identified from the transcriptomic datasets since they possess specific extracellular targeting signals.


Recent Grants and Fellowships

2008  Swiss National Fundation (SNF) short-term grant

„Identification of plant interacting partners for RXLR effector proteins of the pathogen Phytophthora brassicae”, Visiting Scientist, Host lab: Dr. Felix Mauch, University of Fribourg, Department of Plant Biology, Switzerland

 

2010 – 2012 Hungarian MB08-A mobility grant,

„Identification of genes involved in host specificity and pathogenicity of Armillaria species”, Guest researcher, Host:  Phytopathology group, Biodiversity and Conservation Biology Research Unit, WSL, Swiss Federal Research Institute, Zürcherstrasse 111, 8903 Birmensdorf, Switzerland


Conferences

2010  COST meeting, FP0801, "Established and Emerging Phytophthora: Increasing Threats to Woodland and Forest Ecosystems in Europe", June 28-30th 2010, Viterbo, Italy
Szabo I., Sipos G., & Lakatos F., Incidence of Phytophthora species in declining forests in Hungary

 

2011 XIII. Conference "Root and Butt Rot of Forest Trees" IUFRO Working Party 7.02.01
September 4th- 11th 2011. Firenze and San Martino di Castrozza , Italy
Sipos G., Qi W., Künzli M., Okoniewski M., & Rigling D.,  454 sequencing of transcriptomes for virulent and non-virulent Armillaria ostoyae strains and identification of their secretomes - oral presentation

 

2011 "Fungal development and pathogenesis" British Mycological Society Annual Scientific Meeting, September 13-16th 2011. University of Exeter, Exeter, UK
Sipos G., Qi W., Künzli M., Okoniewski M., & Rigling D., Identification of the differential secretomes for virulent and non-virulent Armillaria ostoyae strains through 454 sequencing and exon-based microarrays

 

2011 COST meeting, FP0801, "Established and Emerging Phytophthora: Increasing Threats to Woodland and Forest Ecosystems in Europe", November 21-22nd 2011, Budapest, Hungary

Sipos G., Molecular perspectives in the plant-pathogen interaction research  - oral presentation

 

2012 Mycology Symposium, January 20th 2012, Zürich, Switzerland,

G. Sipos: "Analysis of the transcriptomes for virulent and non-virulent Armillaria ostoyae strains through 454 sequencing and exon-based microarrays" - oral presentation


Invited speaker / lecturer

2009  November, University of Natural Resources and Applied Life Sciences, Dept of Forest- & Soil Sciences, Vienna, Austria, Lectures in "Fungal Secretion and Pathogenicity", funded by an Erasmus fellowship

 

2010  September 14, Norwegian Forest and Landscape Institute, As, Norway,
"Identification of genes involved in host specificity and pathogenicity of Armillaria species"

 

2012  February 2., Uppsala University, Sweden, "Identification of the differential secretomes for virulent and non-virulent Armillaria strains through 454 sequencing and exon-based microarrays"